PDBe 3aaa

X-ray diffraction
2.2Å resolution

Crystal Structure of Actin capping protein in complex with V-1

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Myotrophin Chain: C
Molecule details ›
Chain: C
Length: 123 amino acids
Theoretical weight: 13.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P58546 (Residues: 1-118; Coverage: 100%)
Gene name: MTPN
Sequence domains: Ankyrin repeats (3 copies)
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
F-actin-capping protein subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 33 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P13127 (Residues: 1-286; Coverage: 100%)
Gene name: CAPZA1
Sequence domains: F-actin capping protein alpha subunit
F-actin-capping protein subunit beta isoforms 1 and 2 Chain: B
Molecule details ›
Chain: B
Length: 277 amino acids
Theoretical weight: 31.4 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14315 (Residues: 1-277; Coverage: 100%)
Gene name: CAPZB
Sequence domains: F-actin capping protein, beta subunit

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 71.424Å b: 87.006Å c: 121.035Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.237
Expression system: Escherichia coli