› 3a77

X-ray diffraction
1.8Å resolution

The crystal structure of phosphorylated IRF-3

Released:
DOI: 10.2210/pdb3a77/pdb
PDB entry 3a77 coloured by chain, front view.
PDB entry 3a77 coloured by chain, side view.
PDB entry 3a77 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ser386 phosphorylation of transcription factor IRF-3 induces dimerization and association with CBP/p300 without overall conformational change.
Takahasi K, Horiuchi M, Fujii K, Nakamura S, Noda NN, Yoneyama M, Fujita T, Inagaki F
Genes Cells 15 901-10 (2010)
PMID: 20604809

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172049 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Interferon regulatory factor 3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 242 amino acids
Theoretical weight: 26.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14653 (Residues: 189-427; Coverage: 56%)
Gene name: IRF3
Sequence domains: Interferon-regulatory factor 3
Structure domains: Tumour Suppressor Smad4

Ligands and Environments

2 bound ligands:
Ligand ACY 2 x ACY
Ligand MPD 4 x MPD
1 modified residue:
Ligand SEP 8 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21212
Unit cell:
a: 163.469Å b: 102.459Å c: 68.921Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.221
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Direct Inhibition of IRF-Dependent Transcriptional Regulatory Mechanisms Associated With Disease.
Antonczyk et al. (2019)
8 more

6 mentions without citation

Interferon Regulatory Factor 9 Structure and Regulation.
Paul et al. (2018)
5 more