PDBe 3a5t

X-ray diffraction
2.8Å resolution

Crystal structure of MafG-DNA complex

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
homo dimer
hetero dimer
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor MafG Chains: A, B
Molecule details ›
Chains: A, B
Length: 107 amino acids
Theoretical weight: 12.52 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O54790 (Residues: 21-123; Coverage: 64%)
Gene name: Mafg
Sequence domains: bZIP Maf transcription factor
5'-D(*CP*TP*GP*AP*TP*GP*AP*GP*TP*CP*AP*GP*CP*AP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.59 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*GP*TP*GP*CP*TP*GP*AP*CP*TP*CP*AP*TP*CP*AP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.58 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2221
Unit cell:
a: 40.364Å b: 185.243Å c: 230.263Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.261 0.294
Expression systems:
  • Escherichia coli
  • Not provided