PDBe 3a1k

X-ray diffraction
2.17Å resolution

Crystal structure of Rhodococcus sp. N771 Amidase

Released:

Function and Biology Details

Reaction catalysed:
A monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidase Chain: A
Molecule details ›
Chain: A
Length: 521 amino acids
Theoretical weight: 54.76 KDa
Source organism: Rhodococcus sp. N-771
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7DKE4 (Residues: 1-521; Coverage: 100%)
Gene name: ami
Sequence domains: Amidase
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P41212
Unit cell:
a: 92.672Å b: 92.672Å c: 162.305Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.232
Expression system: Escherichia coli