PDBe 3zg6

X-ray diffraction
2.2Å resolution

The novel de-long chain fatty acid function of human sirt6

Released:

Function and Biology Details

Reaction catalysed:
NAD(+) + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAD-dependent protein deacetylase sirtuin-6 Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 32.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8N6T7 (Residues: 1-296; Coverage: 83%)
Gene names: SIR2L6, SIRT6
Sequence domains: Sir2 family
Structure domains:
Histone H3.1 Chain: F
Molecule details ›
Chain: F
Length: 12 amino acids
Theoretical weight: 1.62 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 5-14; Coverage: 7%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: R32
Unit cell:
a: 104.891Å b: 104.891Å c: 231.492Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.236
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided