PDBe 3wxf

X-ray diffraction
2.3Å resolution

Crystal structure of CYLD USP domain (C596S E674Q) in complex with Met1-linked diubiquitin


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cylindromatosis (turban tumor syndrome), a Chains: A, C
Molecule details ›
Chains: A, C
Length: 312 amino acids
Theoretical weight: 35.99 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
  • Canonical: E7FEV5 (Residues: 578-780, 850-951; Coverage: 32%)
Gene name: cylda
Sequence domains: Ubiquitin carboxyl-terminal hydrolase
Structure domains: Cysteine proteinases
Ubiquitin Chains: B, D
Molecule details ›
Chains: B, D
Length: 148 amino acids
Theoretical weight: 16.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P0CG48 (Residues: 533-680; Coverage: 22%)
Gene name: UBC
Sequence domains: Ubiquitin family

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P1
Unit cell:
a: 49.628Å b: 65.372Å c: 69.846Å
α: 77.66° β: 89.04° γ: 89.46°
R R work R free
0.189 0.187 0.228
Expression system: Escherichia coli