› 3wso

X-ray diffraction
2.6Å resolution

Crystal structure of the Skp1-FBG3 complex

Released:
DOI: 10.2210/pdb3wso/pdb
PDB entry 3wso coloured by chain, front view.
PDB entry 3wso coloured by chain, side view.
PDB entry 3wso coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Structural Differences between a Glycoprotein Specific F-Box Protein Fbs1 and Its Homologous Protein FBG3.
Kumanomidou T, Nishio K, Takagi K, Nakagawa T, Suzuki A, Yamane T, Tokunaga F, Iwai K, Murakami A, Yoshida Y, Tanaka K, Mizushima T
PLoS One 10 e0140366 (2015)
PMID: 26460611

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159046 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
F-box only protein 44 Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 29.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H4M3 (Residues: 1-255; Coverage: 100%)
Gene names: FBG3, FBX30, FBX44, FBX6A, FBXO44, FBXO6A
Sequence domains:
Structure domains:
S-phase kinase-associated protein 1 Chain: B
Molecule details ›
Chain: B
Length: 166 amino acids
Theoretical weight: 18.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63208 (Residues: 1-163; Coverage: 100%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 34.12Å b: 76.594Å c: 193.939Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.266
Expression system: Escherichia coli

Quick links

Citations

2 review citations

PROTAC targeted protein degraders: the past is prologue.
Békés et al. (2022)
1 more

4 mentions without citation

Glycosylation Promotes the Random Coil to Helix Transition in a Region of a Protist Skp1 Associated with F-Box Binding.
Xu et al. (2018)
3 more