PDBe 3wf4

X-ray diffraction
2.3Å resolution

Crystal structure of human beta-galactosidase mutant I51T in complex with 6S-NBI-DGJ

Released:
Source organism: Homo sapiens
Entry authors: Suzuki H, Ohto U, Shimizu T

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 678 amino acids
Theoretical weight: 76.61 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P16278 (Residues: 24-677; Coverage: 100%)
  • Best match: P16278-3 (Residues: 1-647)
Gene names: ELNR1, GLB1
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21
Unit cell:
a: 95.026Å b: 116.175Å c: 140.68Å
α: 90° β: 92.29° γ: 90°
R-values:
R R work R free
0.182 0.179 0.24
Expression system: Komagataella pastoris