PDBe 3wa4

X-ray diffraction
1.35Å resolution

Grb2 SH2 domain/CD28-derived peptide complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Growth factor receptor-bound protein 2 Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 11.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P62993 (Residues: 60-152; Coverage: 43%)
Gene names: ASH, GRB2
Sequence domains: SH2 domain
Structure domains: SH2 domain
T-cell-specific surface glycoprotein CD28 Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 1.04 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: NEW P10747 (Residues: 189-196; Coverage: 4%)
Gene name: CD28

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P6122
Unit cell:
a: 59.01Å b: 59.01Å c: 117.09Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.176 0.209
Expression systems:
  • Escherichia coli
  • Not provided