PDBe 3w31

X-ray diffraction
2.96Å resolution

Structual basis for the recognition of Ubc13 by the Shigella flexneri effector OspI

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ORF169b Chain: A
Molecule details ›
Chain: A
Length: 220 amino acids
Theoretical weight: 24.12 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VSD5 (Residues: 1-212; Coverage: 100%)
Gene names: CBL21_25250, CBW45_25965, ORF169b, SF_p0209
Ubiquitin-conjugating enzyme E2 N Chain: B
Molecule details ›
Chain: B
Length: 155 amino acids
Theoretical weight: 17.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61088 (Residues: 1-152; Coverage: 100%)
Gene names: BLU, UBE2N
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P321
Unit cell:
a: 119.238Å b: 119.238Å c: 69.985Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.245 0.284
Expression system: Escherichia coli