PDBe 3w12

X-ray diffraction
4.3Å resolution

Insulin receptor ectodomain construct comprising domains L1-CR in complex with high-affinity insulin analogue [D-PRO-B26]-DTI-NH2, alpha-CT peptide(704-719) and FAB 83-7

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 26 amino acids
Theoretical weight: 2.94 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-49; Coverage: 29%)
Gene name: INS
Insulin receptor subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 310 amino acids
Theoretical weight: 35.5 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P06213 (Residues: 28-337; Coverage: 23%)
Gene name: INSR
Sequence domains: Receptor L domain
Insulin receptor subunit alpha Chain: F
Molecule details ›
Chain: F
Length: 16 amino acids
Theoretical weight: 1.92 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P06213 (Residues: 731-746; Coverage: 1%)
  • Best match: P06213-2 (Residues: 731-746)
Gene name: INSR
monoclonal antibody fab 83-7 fragment - heavy chain Chain: C
Molecule details ›
Chain: C
Length: 116 amino acids
Theoretical weight: 12.71 KDa
Source organism: Mus musculus
Expression system: Mus musculus
monoclonal antibody fab 83-7 fragment - light chain Chain: D
Molecule details ›
Chain: D
Length: 114 amino acids
Theoretical weight: 12.68 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P23
Unit cell:
a: 169.488Å b: 169.488Å c: 169.488Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.291 0.289 0.349
Expression systems:
  • Not provided
  • Cricetulus griseus
  • Mus musculus