PDBe 3vxm

X-ray diffraction
2.5Å resolution

The complex between C1-28 TCR and HLA-A24 bound to HIV-1 Nef134-10(2F) peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A-24 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05534 (Residues: 25-298; Coverage: 80%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Protein Nef Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 1.27 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
  • Canonical: Q9YYU3 (Residues: 143-152; Coverage: 5%)
Gene name: nef
C1-28 TCR alpha chain Chain: D
Molecule details ›
Chain: D
Length: 211 amino acids
Theoretical weight: 23.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
C1-28 TCR beta chain Chain: E
Molecule details ›
Chain: E
Length: 244 amino acids
Theoretical weight: 27.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 50.117Å b: 86.489Å c: 234.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.209 0.267
Expression systems:
  • Escherichia coli
  • Not provided