PDBe 3vti

X-ray diffraction
2.56Å resolution

Crystal structure of HypE-HypF complex

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Carbamoyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 761 amino acids
Theoretical weight: 86.87 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RDB0 (Residues: 1-751; Coverage: 100%)
Gene names: HypF, TTE0131
Sequence domains:
Structure domains: Alpha-Beta Plaits
Hydrogenase maturation factor Chains: C, D
Molecule details ›
Chains: C, D
Length: 314 amino acids
Theoretical weight: 33.88 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RDA7 (Residues: 40-351; Coverage: 89%)
Gene names: HypE, TTE0134
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 118.739Å b: 119.101Å c: 174.631Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.251
Expression system: Escherichia coli