› 3vkw

X-ray diffraction
1.9Å resolution

Crystal Structure of the Superfamily 1 Helicase from Tomato Mosaic Virus

Released:
DOI: 10.2210/pdb3vkw/pdb
PDB entry 3vkw coloured by chain, front view.
PDB entry 3vkw coloured by chain, side view.
PDB entry 3vkw coloured by chain, top view.
Source organism: Tobacco mosaic virus strain tomato/L
Primary publication:
Crystal structure of the superfamily 1 helicase from Tomato mosaic virus.
Nishikiori M, Sugiyama S, Xiang H, Niiyama M, Ishibashi K, Inoue T, Ishikawa M, Matsumura H, Katoh E
J Virol 86 7565-76 (2012)
PMID: 22573863

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137121 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replicase small subunit Chain: A
Molecule details ›
Chain: A
Length: 446 amino acids
Theoretical weight: 50.03 KDa
Source organism: Tobacco mosaic virus strain tomato/L
Expression system: Escherichia coli
UniProt:
  • Canonical: P03587 (Residues: 666-1111; Coverage: 28%)
Sequence domains: Viral (Superfamily 1) RNA helicase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P212121
Unit cell:
a: 85.441Å b: 128.817Å c: 40.742Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.227
Expression system: Escherichia coli

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Citations

8 review citations

Insights into SARS-CoV-2 genome, structure, evolution, pathogenesis and therapies: Structural genomics approach.
Naqvi et al. (2020)
7 more

10 mentions without citation

Review on Structures of Pesticide Targets.
Li et al. (2020)
9 more