PDBe 3uyo

X-ray diffraction
1.83Å resolution

Crystal structure of monobody SH13/ABL1 SH2 domain complex

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase ABL1 Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00519 (Residues: 112-232; Coverage: 11%)
Gene names: ABL, ABL1, JTK7
Sequence domains: SH2 domain
Structure domains: SH2 domain
Monobody SH13 Chain: D
Molecule details ›
Chain: D
Length: 95 amino acids
Theoretical weight: 10.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P21212
Unit cell:
a: 65.553Å b: 49.181Å c: 60.955Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.237
Expression system: Escherichia coli