PDBe 3ui2

X-ray diffraction
3.18Å resolution

Crystal structure of the cpSRP54 tail bound to cpSRP43

Released:
Source organism: Arabidopsis thaliana
Primary publication:
Chromodomains read the arginine code of post-translational targeting.
Nat. Struct. Mol. Biol. 19 260-3 (2012)
PMID: 22231402

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Signal recognition particle 43 kDa protein, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 26.84 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: O22265 (Residues: 84-327; Coverage: 65%)
Gene names: At2g47450, CAO, CPSRP43, T30B22.25
Sequence domains: Ankyrin repeat
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Signal recognition particle 54 kDa protein, chloroplastic Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.5 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: P37107 (Residues: 528-540; Coverage: 2%)
Gene names: At5g03940, CPSRP54, F8F6_150, FFC

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 112.23Å b: 48.52Å c: 57.85Å
α: 90° β: 90.13° γ: 90°
R-values:
R R work R free
0.21 0.207 0.268
Expression system: Escherichia coli