PDBe 3ueo

X-ray diffraction
2.6Å resolution

Crystal structure of TopBP1 BRCT4/5 domains in complex with a phospho-peptide


Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mediator of DNA damage checkpoint protein 1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 12 amino acids
Theoretical weight: 1.52 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: Q14676 (Residues: 325-336; Coverage: 1%)
Gene names: KIAA0170, MDC1, NFBD1
DNA topoisomerase 2-binding protein 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 203 amino acids
Theoretical weight: 22.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
  • Canonical: Q92547 (Residues: 549-746; Coverage: 13%)
Gene names: KIAA0259, TOPBP1
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P1
Unit cell:
a: 58.81Å b: 59.1Å c: 78.31Å
α: 102.05° β: 98.04° γ: 114.34°
R R work R free
0.192 0.19 0.234
Expression systems:
  • Not provided
  • Escherichia coli BL21