PDBe 3u88

X-ray diffraction
3Å resolution

Crystal structure of human menin in complex with MLL1 and LEDGF

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Menin Chains: A, B
Molecule details ›
Chains: A, B
Length: 550 amino acids
Theoretical weight: 61.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00255 (Residues: 2-615; Coverage: 89%)
  • Best match: O00255-2 (Residues: 2-610)
Gene names: MEN1, SCG2
Sequence domains: Menin
MLL cleavage product N320 Chains: M, N
Molecule details ›
Chains: M, N
Length: 75 amino acids
Theoretical weight: 7.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03164 (Residues: 103-153; Coverage: 1%)
Gene names: ALL1, CXXC7, HRX, HTRX, KMT2A, MLL, MLL1, TRX1
PC4 and SFRS1-interacting protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 89 amino acids
Theoretical weight: 10.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75475 (Residues: 347-435; Coverage: 17%)
Gene names: DFS70, LEDGF, PSIP1, PSIP2
Structure domains: Conserved domain common to transcription factors TFIIS, elongin A, CRSP70

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P6122
Unit cell:
a: 187.99Å b: 187.99Å c: 238.417Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.202 0.233
Expression system: Escherichia coli BL21(DE3)