PDBe 3u3d

X-ray diffraction
2.4Å resolution

Plasmodium falciparum Sir2A preferentially hydrolyzes medium and long chain fatty acyl lysine

Released:

Function and Biology Details

Reaction catalysed:
N(6)-acyl-L-lysine + H(2)O = a carboxylate + L-lysine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAD-dependent protein deacylase Sir2A Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 32.05 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IE47 (Residues: 1-273; Coverage: 100%)
Gene names: PF13_0152, Sir2, Sir2A
Sequence domains: Sir2 family
Structure domains:
Histone H3.1 Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.62 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 5-14; Coverage: 7%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21212
Unit cell:
a: 32.511Å b: 103.328Å c: 105.512Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.276
Expression systems:
  • Escherichia coli
  • Not provided