PDBe 3ts8

X-ray diffraction
2.8Å resolution

Crystal structure of a multidomain human p53 tetramer bound to the natural CDKN1A(p21) p53-response element

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Cellular tumor antigen p53 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 234 amino acids
Theoretical weight: 26.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04637 (Residues: 94-356; Coverage: 60%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like
CDKN1A(p21) sense strand Chain: K
Molecule details ›
Chain: K
Length: 26 nucleotides
Theoretical weight: 8.03 KDa
Source organism: Homo sapiens
Expression system: Not provided
CDKN1A(p21) anti-sense strand Chain: L
Molecule details ›
Chain: L
Length: 26 nucleotides
Theoretical weight: 7.94 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21212
Unit cell:
a: 163.81Å b: 169.203Å c: 55.261Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.236 0.283
Expression systems:
  • Escherichia coli
  • Not provided