PDBe 3tj5

X-ray diffraction
1.99Å resolution

human vinculin head domain (Vh1, residues 1-258) in complex with the vinculin binding site of the surface cell antigen 4 (sca4-VBS-N; residues 412-434) from Rickettsia rickettsii

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vinculin Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 28.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18206 (Residues: 1-255; Coverage: 23%)
Gene name: VCL
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)
Antigenic heat-stable 120 kDa protein Chain: B
Molecule details ›
Chain: B
Length: 27 amino acids
Theoretical weight: 2.81 KDa
Source organism: Rickettsia rickettsii str. Iowa
Expression system: Escherichia coli
UniProt:
  • Canonical: B0BXR4 (Residues: 412-434; Coverage: 2%)
Gene name: RrIowa_0797

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1, null
Spacegroup: P212121
Unit cell:
a: 54.404Å b: 76.343Å c: 114.549Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.194 0.218
Expression system: Escherichia coli