PDBe 3tj3

X-ray diffraction
2.7Å resolution

Structure of importin a5 bound to the N-terminus of Nup50

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-5, N-terminally processed Chains: A, B
Molecule details ›
Chains: A, B
Length: 447 amino acids
Theoretical weight: 49.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52294 (Residues: 66-512; Coverage: 83%)
Gene names: KPNA1, RCH2
Sequence domains: Armadillo/beta-catenin-like repeat
Structure domains: Leucine-rich Repeat Variant
Nuclear pore complex protein Nup50 Chains: C, D
Molecule details ›
Chains: C, D
Length: 114 amino acids
Theoretical weight: 11.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UKX7 (Residues: 1-109; Coverage: 23%)
Gene names: NPAP60L, NUP50, PRO1146
Sequence domains: NUP50 (Nucleoporin 50 kDa)

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A, NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 81.683Å b: 98.291Å c: 135.352Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.255
Expression system: Escherichia coli