PDBe 3thm

X-ray diffraction
2.1Å resolution

Crystal structure of Fas receptor extracellular domain in complex with Fab EP6b_B01

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab EP6b_B01, light chain Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 23.25 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Fab EP6b_B01, heavy chain Chain: H
Molecule details ›
Chain: H
Length: 245 amino acids
Theoretical weight: 26.08 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Tumor necrosis factor receptor superfamily member 6 Chain: F
Molecule details ›
Chain: F
Length: 156 amino acids
Theoretical weight: 17.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P25445 (Residues: 17-172; Coverage: 47%)
Gene names: APT1, FAS, FAS1, TNFRSF6
Sequence domains: TNFR/NGFR cysteine-rich region
Structure domains: Tumor Necrosis Factor Receptor, subunit A, domain 2

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3121
Unit cell:
a: 94.51Å b: 94.51Å c: 139.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.183 0.221
Expression systems:
  • Cricetulus griseus
  • Escherichia coli