PDBe 3t6r

X-ray diffraction
1.95Å resolution

Structure of UHRF1 in complex with unmodified H3 N-terminal tail


Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase UHRF1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 72 amino acids
Theoretical weight: 8.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q96T88 (Residues: 299-364; Coverage: 8%)
Gene names: ICBP90, NP95, RNF106, UHRF1
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
Histone H3.1t Chain: D
Molecule details ›
Chain: D
Length: 7 amino acids
Theoretical weight: 777 Da
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: Q16695 (Residues: 2-8; Coverage: 5%)
Gene names: H3FT, HIST3H3

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P43212
Unit cell:
a: 41.028Å b: 41.028Å c: 183.01Å
α: 90° β: 90° γ: 90°
R R work R free
0.197 0.194 0.229
Expression systems:
  • Escherichia coli
  • Not provided