PDBe 3t4n

X-ray diffraction
2.3Å resolution

Structure of the regulatory fragment of Saccharomyces cerevisiae AMPK in complex with ADP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Carbon catabolite-derepressing protein kinase Chain: A
Molecule details ›
Chain: A
Length: 179 amino acids
Theoretical weight: 20.45 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P06782 (Residues: 457-633; Coverage: 28%)
Gene names: CAT1, CCR1, D8035.20, GLC2, PAS14, SNF1, YDR477W
Sequence domains: Adenylate sensor of SNF1-like protein kinase
Structure domains: Kinase associated domain 1, KA1
SNF1 protein kinase subunit beta-2 Chain: B
Molecule details ›
Chain: B
Length: 113 amino acids
Theoretical weight: 13.04 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P34164 (Residues: 303-415; Coverage: 27%)
Gene names: G1155, SIP2, SPM2, YGL208W
Sequence domains: 5'-AMP-activated protein kinase beta subunit, interaction domain
Structure domains: N-terminal domain of TfIIb
5'-AMP-activated protein kinase subunit gamma Chain: C
Molecule details ›
Chain: C
Length: 323 amino acids
Theoretical weight: 36.51 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P12904 (Residues: 2-322; Coverage: 100%)
Gene names: CAT3, SCI1, SNF4, YGL115W
Sequence domains: CBS domain
Structure domains: CBS-domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2221
Unit cell:
a: 88.599Å b: 251.214Å c: 79.589Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.268
Expression system: Escherichia coli