› 3t0q

X-ray diffraction
2.35Å resolution

Motor Domain Structure of the Kar3-like kinesin from Ashbya gossypii

Released:
DOI: 10.2210/pdb3t0q/pdb
PDB entry 3t0q coloured by chain, front view.
PDB entry 3t0q coloured by chain, side view.
PDB entry 3t0q coloured by chain, top view.
Source organism: Eremothecium gossypii ATCC 10895
Primary publication:
Crystal structure of the Kar3-like kinesin motor domain from the filamentous fungus Ashbya gossypii.
Duan D, Hnatchuk DJ, Brenner J, Davis D, Allingham JS
Proteins 80 1016-27 (2012)
PMID: 22493778

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181095 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 39.91 KDa
Source organism: Eremothecium gossypii ATCC 10895
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q74ZE6 (Residues: 363-709; Coverage: 49%)
Gene name: AGOS_AGR253W
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 44.866Å b: 84.623Å c: 101.141Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 citation in other articles

KIF14 binds tightly to microtubules and adopts a rigor-like conformation.
Arora et al. (2014)
5 more

1 mention without citation

Kinesin Motor Enzymology: Chemistry, Structure, and Physics of Nanoscale Molecular Machines.
Cochran JC. (2015)