PDBe 3t04

X-ray diffraction
2.1Å resolution

Crystal structure of monobody 7c12/abl1 sh2 domain complex

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase ABL1 Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00519 (Residues: 112-232; Coverage: 11%)
Gene names: ABL, ABL1, JTK7
Sequence domains: SH2 domain
Structure domains: SH2 domain
MONOBODY 7C12 Chain: D
Molecule details ›
Chain: D
Length: 103 amino acids
Theoretical weight: 11.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 39.336Å b: 49.396Å c: 107.354Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.251
Expression system: Escherichia coli BL21(DE3)