PDBe 3sqh

X-ray diffraction
2.2Å resolution

Crystal structure of prethrombin-2 mutant S195A in the the open form

Released:

Function and Biology Details

Reaction catalysed:
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thrombin heavy chain Chain: E
Molecule details ›
Chain: E
Length: 290 amino acids
Theoretical weight: 33.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00734 (Residues: 333-622; Coverage: 49%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 44.359Å b: 60.043Å c: 49.691Å
α: 90° β: 96.47° γ: 90°
R-values:
R R work R free
0.192 0.189 0.244
Expression system: Escherichia coli BL21(DE3)