PDBe 3sq2

X-ray diffraction
2.1Å resolution

RB69 DNA Polymerase Ternary Complex with dTTP Opposite 2AP (AT rich sequence)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-directed DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 902 amino acids
Theoretical weight: 104.51 KDa
Source organism: Enterobacteria phage RB69
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38087 (Residues: 1-902; Coverage: 100%)
Gene name: 43
Sequence domains:
Structure domains:
5'-D(P*CP*(2PR)P*TP*AP*AP*TP*TP*AP*AP*TP*TP*AP*AP*TP*TP*G)-3' Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Enterobacteria phage RB69
Expression system: Not provided
5'-D(*AP*AP*TP*TP*AP*AP*TP*TP*AP*AP*TP*TP*(2DA))-3' Chain: P
Molecule details ›
Chain: P
Length: 13 nucleotides
Theoretical weight: 3.96 KDa
Source organism: Enterobacteria phage RB69
Expression system: Not provided

Ligands and Environments

2 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 74.946Å b: 119.884Å c: 130.426Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.244
Expression systems:
  • Escherichia coli
  • Not provided