PDBe 3sjv

X-ray diffraction
3.1Å resolution

Crystal structure of the RL42 TCR in complex with HLA-B8-FLR

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero 20-mer (preferred)
hetero dimer
hetero trimer
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, B-8 alpha chain Chains: A, F, K, P
Molecule details ›
Chains: A, F, K, P
Length: 277 amino acids
Theoretical weight: 32.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30460 (Residues: 25-301; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, G, L, Q
Molecule details ›
Chains: B, G, L, Q
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Epstein-Barr nuclear antigen 3 Chains: C, H, M, R
Molecule details ›
Chains: C, H, M, R
Length: 9 amino acids
Theoretical weight: 1.05 KDa
Source organism: Human gammaherpesvirus 4
Expression system: Not provided
UniProt:
  • Canonical: Q3KST2 (Residues: 325-333; Coverage: 1%)
Gene names: BLRF3-BERF1, EBNA3
T cell receptor alpha constant Chains: D, I, N, S
Molecule details ›
Chains: D, I, N, S
Length: 203 amino acids
Theoretical weight: 22.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01848 (Residues: 1-93; Coverage: 66%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
RL42 T cell receptor, beta chain Chains: E, J, O, T
Molecule details ›
Chains: E, J, O, T
Length: 244 amino acids
Theoretical weight: 27.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 100.08Å b: 185Å c: 217.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.253 0.321
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided
  • Escherichia coli BL21