PDBe 3si8

X-ray diffraction
2.15Å resolution

Human DNA polymerase eta - DNA ternary complex with the 5'T of a CPD in the active site (TT2)


Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase eta Chain: A
Molecule details ›
Chain: A
Length: 435 amino acids
Theoretical weight: 48.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q9Y253 (Residues: 1-432; Coverage: 61%)
Gene names: POLH, RAD30, RAD30A, XPV
Sequence domains: impB/mucB/samB family
Structure domains: Dna Ligase; domain 1
DNA (5'-D(*TP*GP*CP*GP*TP*CP*AP*TP*A)-3') Chain: P
Molecule details ›
Chain: P
Length: 9 nucleotides
Theoretical weight: 2.73 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*A*AP*CP*(TTD)P*AP*TP*GP*AP*CP*GP*C)-3') Chain: T
Molecule details ›
Chain: T
Length: 12 nucleotides
Theoretical weight: 3.95 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P212121
Unit cell:
a: 61.236Å b: 80.259Å c: 139.759Å
α: 90° β: 90° γ: 90°
R R work R free
0.245 0.241 0.258
Expression systems:
  • Escherichia coli
  • Not provided