PDBe 3shw

X-ray diffraction
2.9Å resolution

Crystal structure of ZO-1 PDZ3-SH3-Guk supramodule complex with Connexin-45 peptide

Released:
Source organism: Homo sapiens
Entry authors: Yu J, Pan L, Chen J, Yu H, Zhang M

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tight junction protein ZO-1 Chain: A
Molecule details ›
Chain: A
Length: 468 amino acids
Theoretical weight: 53.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07157 (Residues: 421-888; Coverage: 27%)
Gene names: TJP1, ZO1
Sequence domains:
Structure domains:
Gap junction gamma-1 protein Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P36383 (Residues: 387-396; Coverage: 3%)
Gene names: GJA7, GJC1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 49.005Å b: 86.955Å c: 141.401Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.253
Expression systems:
  • Escherichia coli
  • Not provided