PDBe 3s7k

X-ray diffraction
1.9Å resolution

Structure of thrombin mutant Y225P in the E form

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thrombin light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 35 amino acids
Theoretical weight: 4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00734 (Residues: 329-363; Coverage: 6%)
Gene name: F2
Thrombin heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 259 amino acids
Theoretical weight: 29.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00734 (Residues: 364-622; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21212
Unit cell:
a: 61.937Å b: 86.813Å c: 100.968Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.217
Expression system: Escherichia coli BL21(DE3)