PDBe 3rrm

X-ray diffraction
2.9Å resolution

S. cerevisiae dbp5 l327v bound to nup159, gle1 h337r, ip6 and adp

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Nucleoporin GLE1 Chain: B
Molecule details ›
Chain: B
Length: 297 amino acids
Theoretical weight: 34.27 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12315 (Residues: 244-538; Coverage: 55%)
Gene names: BRR3, D1049, GLE1, RSS1, YDL207W
Sequence domains: GLE1-like protein
Nucleoporin NUP159 Chain: C
Molecule details ›
Chain: C
Length: 388 amino acids
Theoretical weight: 43.04 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40477 (Residues: 2-387; Coverage: 26%)
Gene names: NUP158, NUP159, RAT7, YIL115C
Sequence domains: Nucleoporin or Nuclear pore complex subunit NUP214=Nup159
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
ATP-dependent RNA helicase DBP5 Chain: A
Molecule details ›
Chain: A
Length: 395 amino acids
Theoretical weight: 44.17 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20449 (Residues: 91-482; Coverage: 81%)
Gene names: DBP5, RAT8, YOR046C
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 186.912Å b: 67.982Å c: 132.392Å
α: 90° β: 127.52° γ: 90°
R-values:
R R work R free
0.23 0.228 0.261
Expression system: Escherichia coli BL21(DE3)