PDBe 3rov

X-ray diffraction
2.3Å resolution

Insulin's biosynthesis and activity have opposing structural requirements: a new factor in neonatal diabetes mellitus

Released:
Source organism: Homo sapiens
Entry authors: Weiss MA, Wan ZL, Dodson EJ, Liu M, Xu B, Hua QX, Turkenburg M, Whittingham J, Nakagawa SH, Huang K, Hu SQ, Jia WH, Wang SH, Brange J, Whittaker J, Arvan P, Katsoyannis PG, Dodson GG

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero 12-mer
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 30 amino acids
Theoretical weight: 3.46 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 45.662Å b: 61.772Å c: 46.038Å
α: 90° β: 105.5° γ: 90°
R-values:
R R work R free
0.218 0.218 0.307
Expression system: Not provided