PDBe 3rmu

X-ray diffraction
1.8Å resolution

Crystal structure of human Methylmalonyl-CoA epimerase, MCEE

Released:
Source organism: Homo sapiens
Entry authors: Chaikuad A, Krysztofinska E, Froese DS, Yue WW, Vollmar M, Muniz JRC, von Delft F, Weigelt J, Arrowsmith CH, Edwards AM, Bountra C, Oppermann U, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed:
(R)-methylmalonyl-CoA = (S)-methylmalonyl-CoA
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylmalonyl-CoA epimerase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 134 amino acids
Theoretical weight: 14.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96PE7 (Residues: 45-176; Coverage: 75%)
Gene name: MCEE
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 53.12Å b: 66.69Å c: 76.98Å
α: 90° β: 90.1° γ: 90°
R-values:
R R work R free
0.19 0.187 0.237
Expression system: Escherichia coli BL21(DE3)