PDBe 3rl0

X-ray diffraction
3.8Å resolution

Truncated SNARE complex with complexin (P1)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Vesicle-associated membrane protein 2 Chains: A, E, I, M, Q, U, Y, c
Molecule details ›
Chains: A, E, I, M, Q, U, Y, c
Length: 37 amino acids
Theoretical weight: 4.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P63027 (Residues: 28-60; Coverage: 28%)
Gene names: SYB2, VAMP2
Syntaxin-1A Chains: B, F, J, N, R, V, Z, d
Molecule details ›
Chains: B, F, J, N, R, V, Z, d
Length: 65 amino acids
Theoretical weight: 7.55 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P32851 (Residues: 191-253; Coverage: 22%)
Gene names: Sap, Stx1a
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chains: C, G, K, O, S, W, a, e
Molecule details ›
Chains: C, G, K, O, S, W, a, e
Length: 81 amino acids
Theoretical weight: 9.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt: Gene names: SNAP, SNAP25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chains: D, H, L, P, T, X, b, f
Molecule details ›
Chains: D, H, L, P, T, X, b, f
Length: 65 amino acids
Theoretical weight: 7.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P60880 (Residues: 141-203; Coverage: 31%)
Gene names: SNAP, SNAP25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Complexin-1 Chains: g, h, i, j, k, l, m, n
Molecule details ›
Chains: g, h, i, j, k, l, m, n
Length: 63 amino acids
Theoretical weight: 7.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW O14810 (Residues: 26-83; Coverage: 43%)
Gene name: CPLX1
Structure domains: Single Helix bin

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 53.745Å b: 127.357Å c: 142.725Å
α: 107.49° β: 90.01° γ: 90.05°
R-values:
R R work R free
0.308 0.306 0.345
Expression system: Escherichia coli