PDBe 3rko

X-ray diffraction
3Å resolution

Crystal structure of the membrane domain of respiratory complex I from E. coli at 3.0 angstrom resolution

Released:
Source organism: Escherichia coli BL21(DE3)
Primary publication:
Structure of the membrane domain of respiratory complex I.
Nature 476 414-20 (2011)
PMID: 21822288

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Proton-translocating NADH-quinone oxidoreductase, chain L Chains: B, L
Molecule details ›
Chains: B, L
Length: 613 amino acids
Theoretical weight: 66.51 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N7P6 (Residues: 1-613; Coverage: 100%)
Gene name: ECBD_1383
Sequence domains:
Proton-translocating NADH-quinone oxidoreductase, chain M Chains: C, M
Molecule details ›
Chains: C, M
Length: 509 amino acids
Theoretical weight: 56.56 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N571 (Residues: 1-509; Coverage: 100%)
Gene name: ECBD_1384
Sequence domains: Proton-conducting membrane transporter
NADH-quinone oxidoreductase subunit N Chains: D, N
Molecule details ›
Chains: D, N
Length: 485 amino acids
Theoretical weight: 52.07 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N755 (Residues: 1-485; Coverage: 100%)
Gene names: ECBD_1385, nuoN
Sequence domains: Proton-conducting membrane transporter
NADH-quinone oxidoreductase subunit K Chains: G, K
Molecule details ›
Chains: G, K
Length: 100 amino acids
Theoretical weight: 10.85 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C5W716 (Residues: 1-100; Coverage: 100%)
Gene names: B21_02164, ECBD_1382, ECD_02204, nuoK
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 4L
NADH-quinone oxidoreductase subunit A Chains: A, E
Molecule details ›
Chains: A, E
Length: 147 amino acids
Theoretical weight: 16.47 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N7N4 (Residues: 1-147; Coverage: 100%)
Gene names: ECBD_1373, nuoA
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
NADH-quinone oxidoreductase subunit J Chains: F, J
Molecule details ›
Chains: F, J
Length: 184 amino acids
Theoretical weight: 19.89 KDa
Source organism: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A140N7W8 (Residues: 1-184; Coverage: 100%)
Gene name: ECBD_1381
Sequence domains: NADH-ubiquinone/plastoquinone oxidoreductase chain 6
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 94.98Å b: 116.57Å c: 191.37Å
α: 98.51° β: 104.08° γ: 108.51°
R-values:
R R work R free
0.233 0.232 0.282