PDBe 3rk3

X-ray diffraction
3.5Å resolution

Truncated SNARE complex with complexin

Released:
Source organisms:
Primary publication:
Complexin cross-links prefusion SNAREs into a zigzag array.
OpenAccess logo Nat. Struct. Mol. Biol. 18 927-33 (2011)
PMID: 21785414

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Vesicle-associated membrane protein 2 Chain: A
Molecule details ›
Chain: A
Length: 37 amino acids
Theoretical weight: 4.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P63027 (Residues: 28-60; Coverage: 28%)
Gene names: SYB2, VAMP2
Syntaxin-1A Chain: B
Molecule details ›
Chain: B
Length: 65 amino acids
Theoretical weight: 7.55 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P32851 (Residues: 191-253; Coverage: 22%)
Gene names: Sap, Stx1a
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chain: C
Molecule details ›
Chain: C
Length: 81 amino acids
Theoretical weight: 9.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt: Gene names: SNAP, SNAP25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chain: D
Molecule details ›
Chain: D
Length: 65 amino acids
Theoretical weight: 7.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P60880 (Residues: 141-203; Coverage: 31%)
Gene names: SNAP, SNAP25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Complexin-1 Chain: E
Molecule details ›
Chain: E
Length: 63 amino acids
Theoretical weight: 7.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW O14810 (Residues: 26-83; Coverage: 43%)
Gene name: CPLX1
Structure domains: Single Helix bin

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 75.861Å b: 52.729Å c: 128.712Å
α: 90° β: 95.21° γ: 90°
R-values:
R R work R free
0.272 0.27 0.316
Expression system: Escherichia coli