PDBe 3rk2

X-ray diffraction
2.2Å resolution

Truncated SNARE complex

Released:
Source organisms:
Primary publication:
Complexin cross-links prefusion SNAREs into a zigzag array.
OpenAccess logo Nat. Struct. Mol. Biol. 18 927-33 (2011)
PMID: 21785414

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Vesicle-associated membrane protein 2 Chains: A, E
Molecule details ›
Chains: A, E
Length: 37 amino acids
Theoretical weight: 4.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P63027 (Residues: 28-60; Coverage: 28%)
Gene names: SYB2, VAMP2
Syntaxin-1A Chains: B, F
Molecule details ›
Chains: B, F
Length: 65 amino acids
Theoretical weight: 7.55 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P32851 (Residues: 191-253; Coverage: 22%)
Gene names: Sap, Stx1a
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chains: C, G
Molecule details ›
Chains: C, G
Length: 81 amino acids
Theoretical weight: 9.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt: Gene names: SNAP, SNAP25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Synaptosomal-associated protein 25 Chains: D, H
Molecule details ›
Chains: D, H
Length: 65 amino acids
Theoretical weight: 7.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P60880 (Residues: 141-203; Coverage: 31%)
Gene names: SNAP, SNAP25
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 27.627Å b: 39.769Å c: 102.275Å
α: 83.38° β: 89.94° γ: 89.87°
R-values:
R R work R free
0.23 0.227 0.268
Expression system: Escherichia coli