PDBe 3r93

X-ray diffraction
2.06Å resolution

Crystal structure of the chromo domain of M-phase phosphoprotein 8 bound to H3K9Me3 peptide

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
M-phase phosphoprotein 8 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 62 amino acids
Theoretical weight: 7.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99549 (Residues: 55-116; Coverage: 7%)
Gene names: MPHOSPH8, MPP8
Sequence domains: Chromo (CHRromatin Organisation MOdifier) domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Histone H3.2 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 15 amino acids
Theoretical weight: 1.61 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q71DI3 (Residues: 2-16; Coverage: 11%)
Gene names: H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 71.148Å b: 74.005Å c: 72.609Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.273
Expression systems:
  • Escherichia coli
  • Not provided