PDBe 3qwr

X-ray diffraction
3.25Å resolution

Crystal structure of IL-23 in complex with an adnectin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ADNECTIN Chain: D
Molecule details ›
Chain: D
Length: 109 amino acids
Theoretical weight: 12.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Interleukin-12 subunit beta Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.74 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P29460 (Residues: 23-328; Coverage: 100%)
Gene names: IL12B, NKSF2
Sequence domains: Cytokine interleukin-12p40 C-terminus
Structure domains: Immunoglobulins
Interleukin-23 subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 170 amino acids
Theoretical weight: 18.7 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NPF7 (Residues: 20-189; Coverage: 100%)
Gene names: IL23A, SGRF, UNQ2498/PRO5798
Sequence domains: Interleukin 23 subunit alpha

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: I212121
Unit cell:
a: 77.7Å b: 91.7Å c: 225.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.234 0.264
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda