PDBe 3qo2

X-ray diffraction
2.49Å resolution

Structural insights for MPP8 chromodomain interaction with histone H3 lysine 9

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
M-phase phosphoprotein 8 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 64 amino acids
Theoretical weight: 7.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99549 (Residues: 55-116; Coverage: 7%)
Gene names: MPHOSPH8, MPP8
Sequence domains: Chromo (CHRromatin Organisation MOdifier) domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Histone H3.1 Chains: P, Q, R, S
Molecule details ›
Chains: P, Q, R, S
Length: 15 amino acids
Theoretical weight: 1.61 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-16; Coverage: 11%)
Gene names: H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P43212
Unit cell:
a: 53.461Å b: 53.461Å c: 238.792Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.29
Expression systems:
  • Escherichia coli
  • Not provided