› 3qmw

X-ray diffraction
2.5Å resolution

RedJ with PEG molecule bound in the active site

Released:
DOI: 10.2210/pdb3qmw/pdb
PDB entry 3qmw coloured by chain, front view.
PDB entry 3qmw coloured by chain, side view.
PDB entry 3qmw coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
Structure and function of the RedJ protein, a thioesterase from the prodiginine biosynthetic pathway in Streptomyces coelicolor.
Whicher JR, Florova G, Sydor PK, Singh R, Alhamadsheh M, Challis GL, Reynolds KA, Smith JL
J Biol Chem 286 22558-69 (2011)
PMID: 21543318

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyketide synthase thioesterase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 259 amino acids
Theoretical weight: 28.64 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli
UniProt:
  • Canonical: O54157 (Residues: 6-261; Coverage: 91%)
Gene names: SC3F7.14, SCO5894, redJ
Sequence domains: Thioesterase domain
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 155.545Å b: 45.013Å c: 156.403Å
α: 90° β: 109.49° γ: 90°
R-values:
R R work R free
0.218 0.214 0.284
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Polyketide synthase and non-ribosomal peptide synthetase thioesterase selectivity: logic gate or a victim of fate?
Horsman et al. (2016)
6 more

3 mentions without citation

Structure and function of the RedJ protein, a thioesterase from the prodiginine biosynthetic pathway in Streptomyces coelicolor.
Whicher et al. (2011)
2 more