PDBe 3qlp

X-ray diffraction
2.14Å resolution

X-ray structure of the complex between human alpha thrombin and a modified thrombin binding aptamer (mTBA)

Released:
Source organism: Homo sapiens
Primary publication:
Thrombin-aptamer recognition: a revealed ambiguity.
OpenAccess logo Nucleic Acids Res. 39 7858-67 (2011)
PMID: 21715374

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
Thrombin heavy chain Chain: H
Molecule details ›
Chain: H
Length: 259 amino acids
Theoretical weight: 29.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00734 (Residues: 364-622; Coverage: 43%)
Gene name: F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Thrombin light chain Chain: L
Molecule details ›
Chain: L
Length: 36 amino acids
Theoretical weight: 4.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00734 (Residues: 328-363; Coverage: 6%)
Gene name: F2
modified thrombin binding DNA aptamer Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.74 KDa

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: I222
Unit cell:
a: 68.208Å b: 110.574Å c: 120.916Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.183 0.237
Expression system: Escherichia coli