PDBe 3qdg

X-ray diffraction
2.69Å resolution

The complex between TCR DMF5 and human Class I MHC HLA-A2 with the bound MART-1(26-35)(A27L) peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A-2 alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P01892 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Mart-1 (26-35) peptide Chain: C
Molecule details ›
Chain: C
Length: 10 amino acids
Theoretical weight: 985 Da
T cell receptor alpha constant Chain: D
Molecule details ›
Chain: D
Length: 199 amino acids
Theoretical weight: 22.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: NEW P01848 (Residues: 1-89; Coverage: 64%)
Gene names: TCRA, TRAC
Structure domains: Immunoglobulins
DMF5 beta chain Chain: E
Molecule details ›
Chain: E
Length: 242 amino acids
Theoretical weight: 26.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 228.361Å b: 46.554Å c: 86.036Å
α: 90° β: 106.73° γ: 90°
R-values:
R R work R free
0.221 0.218 0.278
Expression system: Escherichia coli