PDBe 3q3f

X-ray diffraction
2.17Å resolution

Engineering Domain-Swapped Binding Interfaces by Mutually Exclusive Folding: Insertion of Ubiquitin into position 103 of Barnase

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease; Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 21.13 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 535-608, 610-614; Coverage: 12%)
  • Canonical: P00648 (Residues: 48-150, 151-151; Coverage: 85%)
  • nullnull
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P3221
Unit cell:
a: 86.872Å b: 86.872Å c: 75.571Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.184 0.233
Expression system: Escherichia coli