PDBe 3q01

X-ray diffraction
2.1Å resolution

An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular tumor antigen p53 Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 26.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04637 (Residues: 94-356; Coverage: 59%)
  • Best match: P04637-5 (Residues: 55-252)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 69.678Å b: 73.817Å c: 106.685Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.25 0.282
Expression system: Escherichia coli BL21(DE3)