PDBe 3puy

X-ray diffraction
3.1Å resolution

Crystal Structure of an outward-facing MBP-Maltose transporter complex bound to AMP-PNP after crystal soaking of the pretranslocation state

Released:
Source organism: Escherichia coli K-12

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Maltose transport system permease protein MalF Chain: F
Molecule details ›
Chain: F
Length: 514 amino acids
Theoretical weight: 57.05 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P02916 (Residues: 1-514; Coverage: 100%)
Gene names: JW3993, b4033, malF
Sequence domains:
Structure domains:
Maltose transport system permease protein MalG Chain: G
Molecule details ›
Chain: G
Length: 296 amino acids
Theoretical weight: 32.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P68183 (Residues: 1-296; Coverage: 100%)
Gene names: JW3992, b4032, malG
Sequence domains: Binding-protein-dependent transport system inner membrane component
Structure domains: MetI-like
Maltose-binding periplasmic protein Chain: E
Molecule details ›
Chain: E
Length: 378 amino acids
Theoretical weight: 41.62 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-396; Coverage: 100%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II
Maltose/maltodextrin import ATP-binding protein MalK Chains: A, B
Molecule details ›
Chains: A, B
Length: 381 amino acids
Theoretical weight: 42.18 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P68187 (Residues: 1-371; Coverage: 100%)
Gene names: JW3995, b4035, malK
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P1
Unit cell:
a: 76.55Å b: 96.283Å c: 112.283Å
α: 86.91° β: 80.87° γ: 74.32°
R-values:
R R work R free
0.216 0.214 0.254
Expression system: Escherichia coli