PDBe 3pt2

X-ray diffraction
2.5Å resolution

Structure of a viral OTU domain protease bound to Ubiquitin

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Putative RNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 187 amino acids
Theoretical weight: 21.44 KDa
Source organism: Crimean-Congo hemorrhagic fever orthonairovirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6TQF5 (Residues: 1-184; Coverage: 5%)
Sequence domains: OTU-like cysteine protease
Structure domains: Cathepsin B; Chain A
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 75 amino acids
Theoretical weight: 8.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG48 (Residues: 609-683; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P6222
Unit cell:
a: 146.01Å b: 146.01Å c: 58.14Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.164 0.224
Expression system: Escherichia coli BL21(DE3)